Epitope Mapping of 2S albumins and Comparison of Ara h 2, Ara h 6 and Ara h 7 from Peanut
نویسندگان
چکیده
منابع مشابه
Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut
The similarities of two major peanut allergens, Ara h 2 and Ara h 6, in molecular size, amino acid sequence, and structure have made it difficult to obtain natural Ara h 6 free of Ara h 2. The objectives of this study were to purify natural Ara h 6 that is essentially free of Ara h 2 and to compare its IgE reactivity and potency in histamine release assays to Ara h 2. Sodium dodecyl sulfate-pol...
متن کاملEffect of Heating and Glycation on the Allergenicity of 2S Albumins (Ara h 2/6) from Peanut
BACKGROUND Peanut allergy is one of the most common and severe food allergies, and processing is known to influence the allergenicity of peanut proteins. We aimed to establish the effect of heating and glycation on the IgE-binding properties and biological activity of 2S albumins (Ara h 2/6) from peanut. METHODOLOGY/PRINCIPAL FINDINGS Native Ara h 2/6 was purified from raw peanuts and heated ...
متن کاملAra h 2 and Ara h 6 have similar allergenic activity and are substantially redundant.
BACKGROUND The moderately homologous (approx. 60%) proteins Ara h 2 and Ara h 6 are the most potent peanut allergens. This study was designed to define the relative individual contributions of Ara h 2 and Ara h 6 to the overall allergenic activity of a crude peanut extract (CPE). METHODS Ara h 2 and Ara h 6 were removed from CPE by gel filtration chromatography. Ara h 2.01, Ara h 2.02 and Ara...
متن کاملIgE cross-reactivity between the major peanut allergen Ara h 2 and the non-homologous allergens Ara h 1 and Ara h 3
BACKGROUND Ara h 1, a vicilin; Ara h 2, a 2S albumin; and Ara h 3, a legumin, are major peanut allergens. Ara h 2 is an important predictor of clinical reactivity to peanut, but cosensitization to all 3 allergens is correlated with the severity of patients' symptoms. OBJECTIVE We investigated whether cosensitization to these 3 allergens is caused by IgE cross-reactivity, despite the fact that...
متن کاملImportance of Ara h 6 in peanut allergy diagnosis
Results Twenty-two children (median age: 6.63 y.o.; IQR: 5.22-10.12) were classified as allergic in the basis of at least two convincing clinical reactions upon ingestion of PNT in the last two years. Sixty-nine children (median age: 8.01 y.o.; IQR: 6.01-11.27) were classified as tolerant on the basis of regular consumption of PNT. Positive SPT with commercial PNT extract was found in 86% of th...
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ژورنال
عنوان ژورنال: Journal of Allergy and Clinical Immunology
سال: 2018
ISSN: 0091-6749
DOI: 10.1016/j.jaci.2017.12.753